Journal: Biology

Article Title: Chemical Composition, Antioxidant Capacity, and Anticancerous Effects against Human Lung Cancer Cells of a Terpenoid-Rich Fraction of Inula viscosa

doi: 10.3390/biology13090687

Figure Lengend Snippet: IVL DCM decreased the viability of A549 cells. ( A ) MTT assay was used to measure the viability of A549 and HDFn cells treated with the indicated concentrations of IVL DCM for 24, 48, or 72 h. Data represents the mean ± SEM of the percent viability of treated cells compared to vehicle-treated cells. Data are the mean of three independent experiments ( n = 3). *** p < 0.001, **** p < 0.0001. ( B ) A549 cells treated with the indicated concentrations of IVL DCM for 24 h were stained with DAPI nuclear stain. DAPI fluorescence was imaged at 20X magnification using a BioTek Cytation 5 reader. ( C ) A549 cells were treated with the indicated concentrations of IVL DCM for 24 h and then stained with crystal violet. The micrographs represent stained cells imaged at 20X magnification by light microscopy using the Invitrogen EVOS ® FL Cell Imaging System.

Article Snippet: A549 human lung adenocarcinoma cells, the most common subtype of NSCLC, which accounts for approximately 85% of lung cancer cases [ ], human normal neonatal fibroblasts (HDFn), SK-OV-3 (human ovarian cancer), MCF-7 (human breast cancer), MDA-MB-231 (human breast cancer), HepG2 (human liver cancer), and HCT116 (human colorectal cancer) cells were obtained from ATCC and cultured at 37 °C and 5% CO 2 in a humidified incubator.

Techniques: MTT Assay, Staining, Fluorescence, Light Microscopy, Imaging

Journal: Cell reports

Article Title: Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells

doi: 10.1016/j.celrep.2020.107711

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Briefly, for each nucleofection, 1 million cells were resuspended in 100 uL of the Amaxa™ NHDF Nucleofector kit (Lonza, Walkersville, MD), containing 3 ug of each of the four episomal plasmids vectors encoding OCT3/4 and p53 shRNA, SOX2 and KLF4, L-MYC and LIN28, and enhanced green fluorescent protein (eGFP) (Addgene, Boston, MA).

Techniques: Recombinant, Concentration Assay, Knock-Out, SYBR Green Assay, Acid Assay, MTT Assay, DNA Purification, Enzyme-linked Immunosorbent Assay, Hydroxyproline Assay, Colorimetric Assay, Binding Assay, Software, Micro-CT, Transmission Assay, Microscopy, Micro-MRI, High Performance Liquid Chromatography

Journal: ACS Omega

Article Title: Elastomeric PU-Pluronic F‑127 Thin Films: Catalyst and Solvent Effects on Microstructure, Mechanical Properties, and Biocompatibility

doi: 10.1021/acsomega.5c11467

Figure Lengend Snippet: Biocompatibility of synthesized films. (a) Cell viability of human dermal fibroblasts (HDFs) assessed by MTT assay after 24 h incubation with film extracts. Data are mean ± SD ( n = 5). (b) Representative micrographs of HDF morphology. Viable cells show a spindle shape, while nonviable cells are rounded. Scale bar = 200 μm (10×). Sample codes: FS DMEM (Negative Control), 10% DMSO (Positive Control), CS (Commercial film extract), LA_BA (Film extract).

Article Snippet: Human dermal fibroblasts (HDFs), specifically normal human neonatal dermal fibroblasts (ATCC, Cat#PCS-201–010, Manassas, VA), were plated at a seeding density of 30% confluence and cultured in fully supplemented Dulbecco’s modified Eagle medium (FS DMEM) supplemented with 10%v/v fetal bovine serum, 100 units/mL penicillin, and 100 μg/mL streptomycin under humidified conditions at 37 °C and 5% CO 2 .

Techniques: Synthesized, MTT Assay, Incubation, Negative Control, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Lysosomal Exocytosis of Olivacine on the Way to Explain Drug Resistance in Cancer Cells

doi: 10.3390/ijms23116119

Figure Lengend Snippet: SRB assay results (GI 50 values) after treatment with olivacine or ellipticine in A549, MCF-7, LoVo, LoVo/DX, CCRF/CEM, and NHDF cells.

Article Snippet: The A549, CCRF/CEM, LoVo, LoVo/DX, and MCF-7 cells were obtained from ECACC, and NHDF cells from LONZA, Belgium.

Techniques: Sulforhodamine B Assay

Journal: International Journal of Molecular Sciences

Article Title: Lysosomal Exocytosis of Olivacine on the Way to Explain Drug Resistance in Cancer Cells

doi: 10.3390/ijms23116119

Figure Lengend Snippet: MTT assay results (IC 50 values) after treatment with olivacine or ellipticine in A549, MCF-7, LoVo, LoVo/DX, CCRF/CEM, and NHDF cells.

Article Snippet: The A549, CCRF/CEM, LoVo, LoVo/DX, and MCF-7 cells were obtained from ECACC, and NHDF cells from LONZA, Belgium.

Techniques: MTT Assay

Journal: International Journal of Molecular Sciences

Article Title: Lysosomal Exocytosis of Olivacine on the Way to Explain Drug Resistance in Cancer Cells

doi: 10.3390/ijms23116119

Figure Lengend Snippet: Comparison of cell morphology after 24 h incubation with olivacine: ( a ) A549 cell line, 2 µM olivacine; ( b ) NHDF cell line, 5 µM olivacine; ( c ) NHDF cell line, 20 µM olivacine.

Article Snippet: The A549, CCRF/CEM, LoVo, LoVo/DX, and MCF-7 cells were obtained from ECACC, and NHDF cells from LONZA, Belgium.

Techniques: Incubation

Journal: Materials

Article Title: Advancing Antimicrobial Textiles: A Comprehensive Study on Combating ESKAPE Pathogens and Ensuring User Safety

doi: 10.3390/ma17020383

Figure Lengend Snippet: ( A ) Dose-response curves of human dermal fibroblast (HDF) cell viability measured by resazurin assay of zinc chloride, silver nitrate, HM4005, HM4007, and tea tree oil solution after 1 day of exposure. Data show mean ± SE. ( B ) Microscopic bright-field images of control HDF and HDF cultures after 1 day of exposure to different concentrations of zinc chloride, silver nitrate, HM4005, HM4007, and tea tree oil solutions. Scale bars = 100 μm.

Article Snippet: Normal human adult dermal fibroblast cells (HDFs; line CCD-1064Sk—ATCC CRL-2076) were used as a model of dermis cells.

Techniques: Resazurin Assay, Control

Journal: Materials

Article Title: Advancing Antimicrobial Textiles: A Comprehensive Study on Combating ESKAPE Pathogens and Ensuring User Safety

doi: 10.3390/ma17020383

Figure Lengend Snippet: Biocompatible assessment of tested antimicrobial agents. ( A ) Images of leachates prepared from textiles coated with the different antimicrobial agents. Human dermal fibroblast (HDF) cell viability measurements using resazurin assay ( B ) and MTT assay ( C ). Whisker plots show HDF cell viability after 1 day of exposure with the suspension from antimicrobial-agent-impregnated textiles from different fabric–media incubation periods. Data show mean ± SE, * p < 0.05, ** p < 0.01, *** p < 0.0002, and **** p < 0.0001, compared to untreated HDF cells.

Article Snippet: Normal human adult dermal fibroblast cells (HDFs; line CCD-1064Sk—ATCC CRL-2076) were used as a model of dermis cells.

Techniques: Resazurin Assay, MTT Assay, Whisker Assay, Suspension, Incubation